The HPLCtool software allows you to predict the retention times and peak widths, simulate chromatograms and optimize resolution for any possible changes in the pH and organic modifier content (i.e. isocratic, pH gradient, organic modifier gradient and combined pH/organic modifier gradient) in Reversed Phase High-Performance Liquid Chromatography (RP HPLC). The simulations can be based on the literature retention parameters or estimated from preliminary experiments.
The HPLCtool was written in the Department of Biopharmaceutics and Pharmacodynamics at the Medical University of Gdańsk.
The HPLCtool software is written in MATLAB version 7.11.0.
In order to install this software:
1. Verify the Matlab Compiler Runtime (MCR) version 7.14 or Matlab 7.11.0 is installed on your computer.
- If you do not have the correct MCR installed on your machine download MCRInstaller 7.14:
- Run the MCRInstaller.exe program and follow the instructions.
2. Download the HPLCtool software and some related files as a zip-file:
Please unzip all the files and into a single folder on your computer.
3. Run hplctool.exe. Depending on your computer it may take up to one ore two minutes before the software actually runs. If Matlab generates an error saying that a certain dll file is missing, please make sure that the MCR directory is added to the system path specified by your computer’s environment variable.
4. Contact us if there are any problems.
5. THE SOFTWARE IS PROVIDED “AS IS”, WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.